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This research was designed to study the inhibitory effect of crud bacteriocin (Staphyloccin, Pyocin) production from MRSA and resistance Pseudomonas aeruginosa which has been isolated from isolated from Baghdad, Iraq samples of different sources (urine and wounds, ear and eye swab) according to biochemical test and vitek 2 system. In vitro assay with the antagonists and their cell-free culture supernatants crud bacteriocin from MRSA and resistance Pseudomonas aeruginosa strains on agar plates showed that the effectively inhibited growth of the yeast (Candida albicans, Candida tropicalis, Candida kefyer).
The results showed that the Minimum Fungicidal Concentrations were 60% and 70% respectively, also the inhibition zone of reached (26 to 24) mm in solid medium.
INTRODUCTION
MATERIALS AND METHODS
Production crud staphyloccin and pyocin
After growing MRSA and Pseudomonas aeruginosa in a Brain-Heart infusion broth and diluting appropriately to a 0.5 McFarland standard (1.5 × 108 CFU/ml), incubated at 37°C for 18 h. Supernatant fluid after centrifuged at 5000x g for 10 min of the isolates were placed into the antimicrobial activity was determined by measuring the diameter of the inhibition zone around the wells. Preparation of cell free extract, the cells was discarded and the cell free extract was filtered using a syringe with 0.2 µm filter. The cell free extract was gently filtered into sterilized test tubes with 0.2 µm acetate cellulose filter [10].
Determining inhibitory effect of staphyloccin and pyocin on Yeast
The antibacterial spectrum of the bacteriocin (pyocin) from P. aeruginosa and (Staphyloccin) from MRSA was determined using the well diffusion method. The supernatant from a 24 h culture of P. aeruginosa and MRSA was filter sterilized by passage through a 0.45 µm pore size membrane filter (PALL Corporation, Mumbai). of the sterile supernatant were placed in 6-mm-diameter wells that had been cut in Sabourad agar plate previously seeded with the indicator yeast, After 12-24 h of incubation, the diameters of the zones of growth inhibition were measured. Antimicrobial activity was expressed in arbitrary units (AU/ml). One AU was defined as the reciprocal of the highest level of dilute on resulting in a clear zone of growth inhibition [10].
RESULTS AND DISCUSSION
Bacteriocin Typing of MRSA among the 25S. S. aureus isolates, four bacterial isolates S4, S12, S16, S19, S23) produced an efficient staphyloccin, identified by wells diffusion method, depending on the widest inhibition zone and the highest sensitive number of the basic indicator isolates S12. These isolates were used as indicator local in bacteriocin typing. Most of these isolates were susceptible to the staphylococcin of the producer isolates, while pyocin production from only five isolates (P1, P7, P9, P21, P26) in the study identified by wells diffusion method, depending on the widest inhibition zone and the highest sensitive number of the basic indicator isolates P26.
Determination of the inhibitory spectrum: Inhibitory activity was detected by techniques
1. Oscar M, Philippe M, Michel PG, Jacques B, Dominique S (2000) Potent synergism of the combination of fluconazole and cyclosporine in Candida albicans. Antimicrobial Agents Chemother 44: 2373-2381.
2. Debbie AH, Quentin LS, Sanders RJ, Gillian EN, Pat JB, et al. (2004) Identification of the dialyzable serum inducer of germtube formation in Candida albicans. Microbiol 150: 3041-3049.
3. Tserkovniak LS, Roi AO, Kurdysh IK (2009) Synthesis of amino acids of Bacillus subtilis IMV V-7023 in the medium with glycerophosphates. Mikrobiol Z 71: 218-232.
4. Cheryl G, Maryam GN, Mark M, Sandy V, Mark SL, et al. (2001) Candida albicans interacts with the septin ring in yeast and hyphal cells. Molecular Biology of the Cell 12: 3538-3549.
5. Boonnaert CJ, Rouxhet PG (2000) Surface of lactic acid bacteria: Relationships between chemical composition and physic-chemical properties. Appl Environ Microbiol 66: 2548-2554.
6. Ndoti-Nembe A, Vu KD, Doucet N, Lacroix M (2015) Antimicrobial effects of essential oils, nisin and irradiation treatments against Listeria monocytogenes on ready-to-eat carrots. J Food Sci 80: M795-M799
7. Hena JV, Sudha SS (2011) Characterization of staphylococcin by peptide mass fingerprinting. Int J Pharm Bio Sci 2: 269-274.
8. Sharma V, Aseri1 GK, Sohal JS, Khare N, Kumar V (2016) Exploration of bacteriocins as potential food preservatives. IJPTB 3: 55-58.
9. Baron EJ, Finegold SM (1990) Bailey & Scott’s: Diagnostic Microbiology. (8th Edn), Mocby Company, USA.
10. Majeed HAL (2004) Identification and immunological study of Candida spp. causing vaginitis. M.Sc. thesis, College of Science for Women, University of Baghdad.
11. Papon N, Courdavault V, Clastre M, Bennett RJ (2013) Emerging and emerged pathogenic Candida species: beyond the Candida albicans paradigm. PLoS Pathog 9: e1003550.
12. Pfaller MA (2012) Antifungal drug resistance: Mechanisms, epidemiology and consequences for treatment. Am J Med 125: S3-S13.
13. Le Lay C, Akerey B, Fliss I, Subirade M, Rouabhia M (2008) Nisin Z inhibits the growth of Candida albicans and its transition from blastospore to hyphal form. J Appl Microbiol 105: 1630-1639.
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