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Introduction:
Oral squamous cell carcinoma (OSCC) comprises a bulk of all the oral
malignancies and is posing a major health problem among the population. It is
an established fact that tumor stroma plays a vital role in tumor progression.
Therefore, methods to detect, quantify, and analyze collagen are of immense
value in this regard. Picro Sirius red, which has the capability to detect thin
fibers, although frequently used, is seldom exploited to the fullest extent.
Aim: Our goal is
not only to identify nature of fibers, but also to assess the fiber hue and the
spatial distribution of different colors in various grades of OSCC and
correlate it with the metastasis of the tumor. The study has also analyzed the
nature of stromal elements along the clear, close and involved surgical margins
of OSCC.
Materials and
methods: Ten cases each of well, moderately and poorly differentiated OSCC
as well as clear, close and involved margins were stained with hematoxylin
eosin and Picro Sirius red staining for evaluation under polarized microscope.
Results: In this
study we found that the birefringence of the collagen fiber changed from orange
red to yellowish green from well to poorly differentiated OSCC. The collagen
fibers in well-differentiated carcinoma revealed polarizing colors of reddish
orange around the tumor islands in the majority of the fields. To the best of
our knowledge is not being studied so far in the English literature.
Conclusion: In
the present study, it has been observed that stromal changes at the invading front
of the tumor islands and with increasing grade of the tumor can be evaluated
more efficiently with the use of Picro Sirius red stain.
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